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Fitc Conjugated, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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CD73‐overexpressing ADSCs regulate macrophage polarization toward the M2 and inhibit NLRP3 inflammasome activation. (A) Flow cytometry results showing macrophage polarization in each group. <t>CD86</t> was detected using FITC‐conjugated antibody, and CD163 was detected using PE‐conjugated antibody. (B) Western blot and quantitative analysis of NLRP3, ASC, and caspase‐1 expression in vitro. (C) Quantitative analysis of Western blot. (D) ELISA analysis of IL‐1β and IL‐18 levels in each group in vitro. Statistical significance is indicated as ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001. All experiments were independently performed in triplicate. The full‐length, uncropped Western blot images are provided in Figure . ELISA data are summarized in Tables and .
Fitc Conjugated Antimouse Cd86, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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CD73‐overexpressing ADSCs regulate macrophage polarization toward the M2 and inhibit NLRP3 inflammasome activation. (A) Flow cytometry results showing macrophage polarization in each group. <t>CD86</t> was detected using FITC‐conjugated antibody, and CD163 was detected using PE‐conjugated antibody. (B) Western blot and quantitative analysis of NLRP3, ASC, and caspase‐1 expression in vitro. (C) Quantitative analysis of Western blot. (D) ELISA analysis of IL‐1β and IL‐18 levels in each group in vitro. Statistical significance is indicated as ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001. All experiments were independently performed in triplicate. The full‐length, uncropped Western blot images are provided in Figure . ELISA data are summarized in Tables and .
Fluorescein Isothiocyanate Fitc Conjugated Goat Anti Rabbit Igg Secondary Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wheat germ agglutinin conjugated to fitc fitc wga  (Santa Cruz Biotechnology)
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CD73‐overexpressing ADSCs regulate macrophage polarization toward the M2 and inhibit NLRP3 inflammasome activation. (A) Flow cytometry results showing macrophage polarization in each group. <t>CD86</t> was detected using FITC‐conjugated antibody, and CD163 was detected using PE‐conjugated antibody. (B) Western blot and quantitative analysis of NLRP3, ASC, and caspase‐1 expression in vitro. (C) Quantitative analysis of Western blot. (D) ELISA analysis of IL‐1β and IL‐18 levels in each group in vitro. Statistical significance is indicated as ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001. All experiments were independently performed in triplicate. The full‐length, uncropped Western blot images are provided in Figure . ELISA data are summarized in Tables and .
Wheat Germ Agglutinin Conjugated To Fitc Fitc Wga, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fitc conjugated anti human cd163 ghi 61 mouse monoclonal igg1  (Santa Cruz Biotechnology)
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CD73‐overexpressing ADSCs regulate macrophage polarization toward the M2 and inhibit NLRP3 inflammasome activation. (A) Flow cytometry results showing macrophage polarization in each group. <t>CD86</t> was detected using FITC‐conjugated antibody, and CD163 was detected using PE‐conjugated antibody. (B) Western blot and quantitative analysis of NLRP3, ASC, and caspase‐1 expression in vitro. (C) Quantitative analysis of Western blot. (D) ELISA analysis of IL‐1β and IL‐18 levels in each group in vitro. Statistical significance is indicated as ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001. All experiments were independently performed in triplicate. The full‐length, uncropped Western blot images are provided in Figure . ELISA data are summarized in Tables and .
Fitc Conjugated Anti Human Cd163 Ghi 61 Mouse Monoclonal Igg1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fitc conjugated anti rabbit  (Jackson Immuno)
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CD73‐overexpressing ADSCs regulate macrophage polarization toward the M2 and inhibit NLRP3 inflammasome activation. (A) Flow cytometry results showing macrophage polarization in each group. <t>CD86</t> was detected using FITC‐conjugated antibody, and CD163 was detected using PE‐conjugated antibody. (B) Western blot and quantitative analysis of NLRP3, ASC, and caspase‐1 expression in vitro. (C) Quantitative analysis of Western blot. (D) ELISA analysis of IL‐1β and IL‐18 levels in each group in vitro. Statistical significance is indicated as ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001. All experiments were independently performed in triplicate. The full‐length, uncropped Western blot images are provided in Figure . ELISA data are summarized in Tables and .
Fitc Conjugated Anti Rabbit, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sa00003  (Proteintech)
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CD73‐overexpressing ADSCs regulate macrophage polarization toward the M2 and inhibit NLRP3 inflammasome activation. (A) Flow cytometry results showing macrophage polarization in each group. <t>CD86</t> was detected using FITC‐conjugated antibody, and CD163 was detected using PE‐conjugated antibody. (B) Western blot and quantitative analysis of NLRP3, ASC, and caspase‐1 expression in vitro. (C) Quantitative analysis of Western blot. (D) ELISA analysis of IL‐1β and IL‐18 levels in each group in vitro. Statistical significance is indicated as ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001. All experiments were independently performed in triplicate. The full‐length, uncropped Western blot images are provided in Figure . ELISA data are summarized in Tables and .
Sa00003, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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CD73‐overexpressing ADSCs regulate macrophage polarization toward the M2 and inhibit NLRP3 inflammasome activation. (A) Flow cytometry results showing macrophage polarization in each group. <t>CD86</t> was detected using FITC‐conjugated antibody, and CD163 was detected using PE‐conjugated antibody. (B) Western blot and quantitative analysis of NLRP3, ASC, and caspase‐1 expression in vitro. (C) Quantitative analysis of Western blot. (D) ELISA analysis of IL‐1β and IL‐18 levels in each group in vitro. Statistical significance is indicated as ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001. All experiments were independently performed in triplicate. The full‐length, uncropped Western blot images are provided in Figure . ELISA data are summarized in Tables and .
Fitc Conjugated Goat Anti Mouse Secondary Antibody, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fitc conjugated goat antirabbit igg  (Proteintech)
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CD73‐overexpressing ADSCs regulate macrophage polarization toward the M2 and inhibit NLRP3 inflammasome activation. (A) Flow cytometry results showing macrophage polarization in each group. <t>CD86</t> was detected using FITC‐conjugated antibody, and CD163 was detected using PE‐conjugated antibody. (B) Western blot and quantitative analysis of NLRP3, ASC, and caspase‐1 expression in vitro. (C) Quantitative analysis of Western blot. (D) ELISA analysis of IL‐1β and IL‐18 levels in each group in vitro. Statistical significance is indicated as ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001. All experiments were independently performed in triplicate. The full‐length, uncropped Western blot images are provided in Figure . ELISA data are summarized in Tables and .
Fitc Conjugated Goat Antirabbit Igg, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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abclonal as011 fitc conjugated anti igg  (ABclonal Biotechnology)
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CD73‐overexpressing ADSCs regulate macrophage polarization toward the M2 and inhibit NLRP3 inflammasome activation. (A) Flow cytometry results showing macrophage polarization in each group. <t>CD86</t> was detected using FITC‐conjugated antibody, and CD163 was detected using PE‐conjugated antibody. (B) Western blot and quantitative analysis of NLRP3, ASC, and caspase‐1 expression in vitro. (C) Quantitative analysis of Western blot. (D) ELISA analysis of IL‐1β and IL‐18 levels in each group in vitro. Statistical significance is indicated as ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001. All experiments were independently performed in triplicate. The full‐length, uncropped Western blot images are provided in Figure . ELISA data are summarized in Tables and .
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CD73‐overexpressing ADSCs regulate macrophage polarization toward the M2 and inhibit NLRP3 inflammasome activation. (A) Flow cytometry results showing macrophage polarization in each group. CD86 was detected using FITC‐conjugated antibody, and CD163 was detected using PE‐conjugated antibody. (B) Western blot and quantitative analysis of NLRP3, ASC, and caspase‐1 expression in vitro. (C) Quantitative analysis of Western blot. (D) ELISA analysis of IL‐1β and IL‐18 levels in each group in vitro. Statistical significance is indicated as ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001. All experiments were independently performed in triplicate. The full‐length, uncropped Western blot images are provided in Figure . ELISA data are summarized in Tables and .

Journal: Stem Cells International

Article Title: CD73‐Overexpressing ADSCs Promote Neurogenic Bladder Repair via A2A–JAK1/STAT3‐Mediated Modulation of Inflammation and Macrophage Polarization

doi: 10.1155/sci/8564160

Figure Lengend Snippet: CD73‐overexpressing ADSCs regulate macrophage polarization toward the M2 and inhibit NLRP3 inflammasome activation. (A) Flow cytometry results showing macrophage polarization in each group. CD86 was detected using FITC‐conjugated antibody, and CD163 was detected using PE‐conjugated antibody. (B) Western blot and quantitative analysis of NLRP3, ASC, and caspase‐1 expression in vitro. (C) Quantitative analysis of Western blot. (D) ELISA analysis of IL‐1β and IL‐18 levels in each group in vitro. Statistical significance is indicated as ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001. All experiments were independently performed in triplicate. The full‐length, uncropped Western blot images are provided in Figure . ELISA data are summarized in Tables and .

Article Snippet: For intracellular marker staining, cells were blocked with 5% bovine serum albumin and incubated for 30 min at room temperature with FITC‐conjugated antimouse CD86 (Elabscience, Wuhan, Hubei, China; 1:100 dilution) and PE‐conjugated antimouse CD163 antibodies (Elabscience, Wuhan, Hubei, China; 1:100 dilution).

Techniques: Activation Assay, Flow Cytometry, Western Blot, Analysis, Expressing, In Vitro, Enzyme-linked Immunosorbent Assay